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sfm4cho medium  (Cytiva Europe)


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    Structured Review

    Cytiva Europe sfm4cho medium
    Sfm4cho Medium, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sfm4cho+medium/pm38561148-42-11-13
    Average 86 stars, based on 1 article reviews
    sfm4cho medium - by Bioz Stars, 2026-11
    86/100 stars

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    Related Articles

    other:

    Article Title: Nature as blueprint: Global phenotype engineering of CHO production cells based on a multi-omics comparison with plasma cells.
    Article Snippet: Especially for the production of artificial, difficult to express molecules a further development of the CHO production cell line is required to keep pace with the continuously increasing demands.. However, the identification of novel targets for cell line engineering to improve CHO cells is a time and cost intensive process.. Since plasma cells are evolutionary optimized for a high antibody expression in mammals, we performed a comprehensive multi-omics comparison between CHO and plasma cells to exploit optimized cellular production traits.

    Cell Culture:

    Article Title: Knockout of a difficult-to-remove CHO host cell protein, lipoprotein lipase, for improved polysorbate stability in monoclonal antibody formulations
    Article Snippet: .. CHO cell culture A null CHO-K1 cell line (ATCC, Manassas, VA, USA) was adapted to serum-free, suspension culture in 125 mL shake flasks containing 20–30 mL SFM4CHO medium (GE Healthcare Life Sciences, Little Chalfont, UK). ..

    Article Title: Exploring synthetic biology for the development of a sensor cell line for automated bioprocess control
    Article Snippet: .. Suspension-adapted CHO-DG44 (A1100001, Thermo Fisher, Darmstadt, Germany) and CHO-K1 (CCL-61TM, ATCC) cells were cultured in chemically defined HyClone SFM4CHO medium (Cytiva, Marlborough, MA, USA) supplemented with 10 g/L glucose (Roth, Karlsruhe, Germany) and 4 mM l -glutamine (Lonza, Basel, Switzerland) at 37 °C, 5% CO 2 , 85% humidity, and 140 rpm (25 mm orbit) in shake flasks (Corning Inc, New York, USA) using a Kuhner incubate shaker (Adolf Kühner AG, Basel, Swiss). ..

    Suspension:

    Article Title: Knockout of a difficult-to-remove CHO host cell protein, lipoprotein lipase, for improved polysorbate stability in monoclonal antibody formulations
    Article Snippet: .. CHO cell culture A null CHO-K1 cell line (ATCC, Manassas, VA, USA) was adapted to serum-free, suspension culture in 125 mL shake flasks containing 20–30 mL SFM4CHO medium (GE Healthcare Life Sciences, Little Chalfont, UK). ..

    Article Title: Knockout of a difficult-to-remove CHO host cell protein, lipoprotein lipase, for improved polysorbate stability in monoclonal antibody formulations.
    Article Snippet: .. A null CHO-K1 cell line (ATCC, Manassas, VA) was adapted to serum-free, suspension culture in 125mL shake flasks containing 20–30mL SFM4CHO medium (GE Healthcare Life Sciences, Little Chalfont, UK). ..

    Article Title: Knockout of a difficult-to-remove CHO host cell protein, lipoprotein lipase, for improved polysorbate stability in monoclonal antibody formulations
    Article Snippet: .. A null CHO-K1 cell line (ATCC, Manassas, VA, USA) was adapted to serum-free, suspension culture in 125 mL shake flasks containing 20–30 mL SFM4CHO medium (GE Healthcare Life Sciences, Little Chalfont, UK). ..

    Article Title: High-efficiency and multilocus targeted integration in CHO cells using CRISPR-mediated donor nicking and DNA repair inhibitors.
    Article Snippet: .. Suspension‐adapted CHO‐K1 cells (ATCC) were cultivated in SFM4CHO medium with L‐glutamine (Cytiva) and passaged at 3‐day intervals. ..

    Article Title: Exploring synthetic biology for the development of a sensor cell line for automated bioprocess control
    Article Snippet: .. Suspension-adapted CHO-DG44 (A1100001, Thermo Fisher, Darmstadt, Germany) and CHO-K1 (CCL-61TM, ATCC) cells were cultured in chemically defined HyClone SFM4CHO medium (Cytiva, Marlborough, MA, USA) supplemented with 10 g/L glucose (Roth, Karlsruhe, Germany) and 4 mM l -glutamine (Lonza, Basel, Switzerland) at 37 °C, 5% CO 2 , 85% humidity, and 140 rpm (25 mm orbit) in shake flasks (Corning Inc, New York, USA) using a Kuhner incubate shaker (Adolf Kühner AG, Basel, Swiss). ..

    Purification:

    Article Title: Recombinant RBD of the SARS-CoV-2 Spike Protein: Production in Escherichia coli Cells, Binding to Antibodies, and Antiviral Activity
    Article Snippet: The aim of the study is to synthesize a recombinant protein in Escherichia coli cells that carries the receptor-binding domain (RBD) of Spike protein of SARS CoV-2, with antiviral activity comparable to the activity of RBD obtained in a eukaryotic cells (eRBD).. 6His-RBDshort (24.4 kDa) and 6His-RBDlong (33.7 kDa) proteins were expressed in Escherichia coli strain BL21 (DE3).. Chromatographic purification of the proteins was carried out on WorkBeads 40 Ni-NTA and WorkBeads 40S sorbents followed by multistage refolding.



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